In this Application Note, multi-parallel screening of binding conditions on CIM® Oligo dT 96-well plates was performed to optimise the dynamic binding capacity (DBC) in the shortest time possible.
What you will learn:
- How to effectively use Monolithic 96-well plates when developing methods. They are unique on the market in combining the standard well-plate design with monolithic technology and Oligo dT for high throughput of mRNA purification.
- Techniques to fine-tune binding conditions and reach higher binding capacities than the non-optimised DBC (2-4 mg/mL) of Oligo dT for mRNA.
- How to effortlessly scale-up results to the standard preparative line of monolithic columns.
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