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Among the most common critical quality attributes (CQAs) for early developability assessment is thermal stability. One particular tool for assessing thermal stability with high resolution is nano-differential scanning fluorimetry (nanoDSF). 

Read this mini-guide to learn: 

  • How nanoDSF uses your protein’s intrinsic fluorescence to determine its melting temperature, Tm and onset of melting, Ton
  • Why high-resolution unfolding data is critical for obtaining stability information about your mAbs
  • What some real-life examples of nanoDSF data look like, and what to look for in your own samples

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