Mitochondrial dysfunction is known to play a central role in drug-induced hepatotoxicity, and thus early detection of mitochondrial toxicity is a critical component of initial drug development efforts. While immortalised cell lines are widely used for in vitro assessment of hepatotoxicity, cryopreserved primary human hepatocytes are a preferred model for drug safety screening.

In this App Note you will learn:

• How Agilent Seahorse technology provides a screening solution that is highly sensitive and direct.
• About the Agilent XF Mito Tox assay as a useful tool in the early detection of mitochondrial toxicity - a critical component of drug development efforts.
• How to use the Agilent Seahorse XF Mito Tox assay with primary human hepatocytes and predict DILI with sensitivity and ease. 
• How to build a streamlined workflow with the Agilent Seahorse XF Mito Tox assay to understand the mitochondrial dysfunction role in drug-induced hepatotoxicity.

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