Flow cytometry is a powerful tool allowing you to detect, measure, and quantify single cells or particles in suspension as they pass through a laser. 

However, to produce impactful results, experiments must be designed with care. This guide takes you through the fundamentals of flow cytometry, provides useful practical advice to optimise your experiments and best practice recommendations when planning multicolour experiments. Also featured are common applications and new technology as well as common protocols. 

This guide is ideal if you are new to flow cytometry, want to expand your knowledge, or as a teaching aide.

Topics covered in the guide include:

• Principles of the Flow Cytometer
• Principles of Fluorescence
• Data Analysis
• Controls in Flow
• Optimising Your Experiments
• Multicolour Panel Building
• Common Applications and New Technology
• Common Protocols
• Troubleshooting